人血管内皮细胞生长因子(VEGF)ELISA Kit,RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor|cusabio.cn

人血管内皮细胞生长因子(VEGF)ELISA Kit

Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT

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试剂盒 > 酶联免疫试剂盒 > 人血管内皮细胞生长因子(VEGF)ELISA Kit

- ELISA Kit
- 人血管内皮细胞生长因子(VEGF)ELISA Kit
- This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.[provided by RefSeq]
- UniProtP15692
- BIOWWWVEGFA
- HGNC12680
- RGD619991
- MGI103178
- OMIM192240
- CUSABIOCSB-E11718h
产品类型:
ELISA Kit
产品名称:
人血管内皮细胞生长因子(VEGF)ELISA Kit
英文名称:
Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT
货号:
CSB-E11718h
别名:
RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
规格:
96T
中文价格:
3600
种属:
Homo sapiens (Human)
其他种属:
Bos taurus (Bovine) Gallus gallus (Chicken) Canis lupus familiaris (Dog) (Canis familiaris) Guinea Sus scrofa (Pig) Equus caballus (Horse) Mus musculus (Mouse) Sus scrofa (Pig) Rattus norvegicus (Rat) Ovis aries (Sheep)
待测物名称:
vascular endothelial growth factor A
缩写:
VEGFA
蛋白功能1:
Cytokine
蛋白功能3:
Angiogenesis
检测范围:
31.25 pg/mL-2000 pg/mL
灵敏度:
25.297 pg/mL
反应时间:
1-5h
所需样本体积:
50-100ul
检测波长:
450 nm
下载:

标准曲线:

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.651	2.751	2.701	2.547
1000	1.729	1.829	1.779	1.625
500	1.067	1.087	1.077	0.923
250	0.579	0.589	0.584	0.430
125	0.372	0.382	0.377	0.223
62.5	0.255	0.265	0.260	0.106
31.25	0.207	0.209	0.208	0.054
0	0.153	0.154	0.154

精密度:

线性度:

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	93
	Range %	86-97
1:4	Average %	87
	Range %	82-93
1:8	Average %	96
	Range %	91-105

回收率:

The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	90-96

定性/定量:
quantitative
研究领域:
Cancer
特异性：
This assay has high sensitivity and excellent specificity for detection of Homo sapiens (Human) VEGFA. No significant cross-reactivity or interference between Homo sapiens (Human) VEGFA and analogues was observed.
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
样本搜集及储存：
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
检测步骤：
Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
结果计算：
Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the VEGFA concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

引用文献:

1. Propranolol given orally for proliferating infantile haemangiomas: analysis of efficacy and serological changes in vascular endothelial growth factor and endothelial nitric oxide synthase in 35 patients , Yuan WL et al,Br J Oral Maxillofac Surg,2013 Jan 3. pii: S0266-4356(12)00623-7

2. Preparation and characterization of decellularized tendon slices for tendon tissue engineering , /,journal of biomedical materials research part a,2012 Jun;100(6):1448-56.

3. Expression and significance of vascular endothelial growth factor A and C in leukemia central nervous system metastasis , Tang YT et al,Leuk Res,2013 Apr;37(4):359-66.

4. The Role of Insulin-Like Growth Factor I and Hypoxia Inducible Factor 1a in Vascular Endothelial Growth Factor Expression in Type 2 Diabetes , Jiang F et al,Ann Clin Lab Sci,2013 Winter;43(1):37-44.

5. The biological effects and mechanisms of calcitonin gene-related peptide on human endothelial cell , Tuo Y et al,J Recept Signal Transduct Res.,2013 Apr;33(2):114-23.

6. Epigenetic silencing of miR-126 contributes to tumor invasion and angiogenesis in colorectal cancer , Zhang Y et al,Oncol Rep,2013 Jul 23.

7. MicroRNA-26a suppresses angiogenesis in human hepatocellular carcinoma by targeting HGF-cMet pathway , Yang X et al,Hepatology,2013 Nov 21.

8. The Association of Serum Vascular Endothelial Growth Factor and Ferritin in Diabetic Microvascular Disease , Guo L et al,Diabetes Technol Ther,2013 Nov 26.

9. Ethyl-p-methoxycinnamate isolated from kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-a, and angiogenesis by blocking endothelial functions. , Umar MI et al,Clinics,2014 Feb;69(2):134-44.

10. Balanced oxidative status by nesfatin-1 in intestinal ischemia-reperfusion , Ceylan Ayada. et al,Int J Clin Exp Med,2015 Mar 15;8(3):3318-24. eCollection 2015.

11. Correlation between vascular endothelial growth factor and subclinical atherosclerosis in patients with psoriasis , Shahidi-Dadras M. et al,Int J Dermatol.,2015 Jul 1.

12. Association of gene polymorphism with serum levels of inflammatory and angiogenic factors in Pakistani patients with age-related macular degeneration , Ambreen F. et al,Mol Vis,2015 Aug 29;21:985-99. eCollection 2015.

13. Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model , Li??in Lin. et al,Molecular Medicine Reports,2015:1070-1076

14. Angiogenic factors are associated with development of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation , Nie DM. et al,J Huazhong Univ Sci Technolog Med Sci,2015,35(5):694-699

15. Levels of Serum 25(OH)VD3, HIF-1伪, VEGF, vWf, and IGF-1 and Their Correlation in Type 2 Diabetes Patients with Different Urine Albumin Creatinine Ratio , Ying Shao.et al, Diabetes Research,2016,7

16. Differing Expression of Cytokines and Tumor Markers in Combined Pulmonary Fibrosis and Emphysema compared to Emphysema and Pulmonary Fibrosis , Xu, Ling.et al,COPD: Journal of Chronic Obstructive Pulmonary Disease ,27 Jan

17. Correlation of Plasma Vascular Endothelial Growth Factor and Endostatin Levels with Symptomatic Intra-and Extracranial Atherosclerotic Stenosis in a Chinese Han , Yu F.et al,J Stroke Cerebrovasc Dis. , Feb 8

18. Levels of Serum 25(OH)VD3, HIF-1α, VEGF, vWf, and IGF-1 and Their Correlation in Type 2 Diabetes Patients with Different Urine Albumin Creatinine Ratio. , Shao Y.et al,J Diabetes Res.,

19. Vitreous and plasma changes of endothelin-1, adrenomedullin and vascular endothelium growth factor in patients with proliferative diabetic retinopathy , Vingolo EM.et al,Eur Rev Med Pharmacol Sci.,Feb;21(4):662-668.

20. IL-17 induces reactive astrocytes and up-regulation of vascular endothelial growth factor (VEGF) through JAK/STAT signaling , You T.et al,Sci Rep. , Mar 10

21. Angiogenic effects of low-intensity cathodal direct current on ischemic diabetic foot ulcers: A randomized controlled trial , Mohammad Reza Asadi.et al,Diabetes Res Clin Pract.,Mar 21;127:147-155.

22. Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia?inducible factor 1α in human dermal fibroblasts , Hui SongCui.et al,Archives of Biochemistry and Biophysics,24 July

23. Exercise training with dietary restriction enhances circulating irisin level associated with increasing endothelial progenitor cell number in obese adults: an intervention study , Huang J.et al,PeerJ.,Aug 14

24. Serum vascular endothelial growth factor in Iranian patients with moderate-severe psoriasis before and after treatment: a PASI-75 response as a practical treatment goal , Mohammad Shahidi-Dadras.et al,Iranian Journal of Dermatolog, Aug

25. Fibroblast activation protein in osteosarcoma cells promotes angiogenesis via AKT and ERK signaling pathways , Chao Zeng.et al,Oncology Letters,Feb 12

26. Assessment of vascular endothelial growth factor in systemic lupus erythematosus patients with anti-phospholipid syndrome , Iman I.El-Gazzar.et al,The Egyptian Rheumatologist,7 April

27. Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification , Qingzhen Zhou.et al,Royal Society of Chemistry,July 19

28. A randomized controlled trial comparing electroacupuncture with manual acupuncture for motor function recovery after ischemic stroke , Chaobo Zhen .et al,European Journal of Integrative Medicine,August 20

29. Synergistic antiproliferative effects of curcumin and celecoxib in hepatocellular carcinoma HepG2 cells , Fatma M.et al,Naunyn-Schmiedeberg\'s Archives of Pharmacology,August 28

30. Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124 , Yin D, et al,Cellular Physiology and Biochemistry,November 9

31. Gac fruit extracts ameliorate proliferation and modulate angiogenic markers of human retinal pigment epithelial cells under high glucose conditions , Ali Abdulqader, et al,Asian Pacific Journal of Tropical Biomedicine,December 26

32. Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes , Liu HN, et al,International Journal of Ophthalmology,December 18

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Title
Expression and significance of vascular endothelial growth factor A and C in leukemia central nervous system metastasis
Author
Tang YT et al
PMID
23137522
Type of publication
Product-specific
Year
2012
Impact Factor
2.923
Journal
Leuk Res
Other information
2013 Apr;37(4):359-66. doi: 10.1016/j.leukres.2012.10.008. Epub 2012 Nov 6.
产品名称货号规格

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.