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        1. 试剂盒 > 酶联免疫试剂盒 > 人叶酸(FA) ELISA Kit
            • ELISA Kit
            • 人叶酸(FA) ELISA Kit
          • 产品类型:
            ELISA Kit
          • 产品名称:

            人叶酸(FA) ELISA Kit

          • 英文名称:

            Human Folic acid(FA) ELISA Kit

          • 货号:
            CSB-E17109h
          • 别名:
            N/A
          • 规格:
            96T
          • 中文价格:
            3600
          • 种属:
            Homo sapiens (Human)
          • 其他种属:
          • 待测物名称:
            Folic acid(FA)
          • 缩写:
            FA
          • 蛋白功能1:
            Metabolism
          • 检测范围:
            12.5 pg/mL-200 pg/mL
          • 灵敏度:
            6.25 pg/mL
          • 反应时间:
            1-5h
          • 所需样本体积:
            50-100ul
          • 检测波长:
            450 nm
          • 下载:
          • 标准曲线:
            These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
            pg/ml OD1 OD2 Average    
            200 0.093 0.090 0.092    
            100 0.227 0.208 0.218    
            50 0.378 0.367 0.373    
            25 0.618 0.637 0.628    
            12.5 0.986 1.003 0.995    
            0 1.485 1.429 1.457    
          • 精密度:
            Intra-assay Precision (Precision within an assay): CV%<8%      
            Three samples of known concentration were tested twenty times on one plate to assess.  
            Inter-assay Precision (Precision between assays): CV%<10%      
            Three samples of known concentration were tested in twenty assays to assess.    
                         
          • 线性度:
            To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
              Sample Serum(n=4)  
            1:20 Average % 89  
            Range % 84-93  
            1:40 Average % 93  
            Range % 89-97  
            1:80 Average % 95  
            Range % 90-100  
            1:160 Average % 84  
            Range % 80-88  
          • 回收率:
            The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
            Sample Type Average % Recovery Range  
            Serum (n=5) 99 94-105  
            EDTA plasma (n=4) 95 90-99  
                         
                         
          • 定性/定量:
            quantitative
          • 研究领域:
            Metabolism
          • 引用文献:
            1. Associations between homocysteine metabolism related SNPs and carotid intima-media thickness: a Chinese sib pair study , Sun K.et al,J Thromb Thrombolysis.,Nov 7
            2. Studying the association between methylene tetrahdrofolate reductase (MTHFR) 677 gene polymorphism, cardiovascular risk and lichen planus , Laila Rashed.et al,Journal of Oral Pathology&Medicine,25 May
            3. Serum homocysteine is associated with polycystic ovarian syndrome in Jordan , Nesreen Saadeh.et al,BIOMEDICAL REPORTS,September 14